Colleen O’Loughlin-Biochemistry

Chemistry and Biochemistry

 

Colleen T. O'Loughlin

Assistant Professor of Chemistry and Biochemistry

Associations

Chemistry and Biochemistry

Dana Chemistry Hall, Room 321

207-786-6390coloughl@bates.edu

About

Education

  • B.S., Biochemistry, Minor in Art, Ithaca College
  • Ph.D., Molecular Biology, Princeton University
  • Postdoctoral Scholar:  Bioengineering, Stanford University

Courses Fall 2018

  • CHEM 107 Atomic and Molecular Structure
  • CHEM 321 Biochemistry Lab

Research Interests

We are decidedly bad at being human on our own. Throughout the course of evolution, we outsourced essential chemistry to the bacteria that reside inside and on our bodies. These bacteria synthesize a variety of small molecules and enzymes that break down our food, educate our immune systems, inhibit the growth of pathogenic bacteria, alter neurotransmitter levels, and potentially carry out thousands of other activities we have yet to identify that could impact human health. My research focuses on identifying molecules made by prominent skin bacteria and understanding how these molecules affect the human host. Once we understand the rules for what chemical signatures enable bacteria to “talk” to us, their human host, we will engineer microbes to synthesize drug-like molecules containing these features. Such “skin probiotics” could possibly mitigate symptoms of skin diseases, including but not limited to, acne and eczema. Research in my laboratory uses techniques from biochemistry, microbiology, analytical chemistry, cell culture, and small-molecule screening.

Publications

5. Tran A., Tang, A., O’Loughlin, C.T., Balistreri, A., Chang, E., Coto Villa, D., Li, J., Varshney, A., Jimenez, V., Pyle, J., Tsujimoto, B., Wellbrook, C., Vargas, C., Duong. A., Ali, N., Matthews, S.Y., Levinson,S., Woldemariam, S., Khuri, S., Bremer, M., Eggers, D.K., L’Etoile, N., Miller Conrad, L.C., Miri VanHoven, M.K., C. elegans avoids toxin-producing Streptomyces using a seven transmembrane domain chemosensory receptor. eLife. 2017; 6: e23770. Sep 5. doi: 10.7554/eLife.23770

4. Miller, L.C., O’Loughlin, C.T., Zhang, Z., Siryaporn, A., Silpe, J.E., Bassler, B.L., Semmelhack, M.F., Development of potent inhibitors of pyocyanin production in Pseudomonas aeruginosa. J Med Chem. 2015 Feb 12; 58(3): 1298–1306. Published online 2015 Feb 3. doi: 10.1021/jm5015082

3. O’Loughlin, C.T., Miller, L.C., Siryaporn, A., Drescher, K., Semmelhack, M.F., Bassler, B.L., A quorum-sensing inhibitor blocks Pseudomonas aeruginosa virulence and biofilm formation. Proc Natl Acad Sci U S A. 2013 Oct 29; 110(44): 17981–17986. Published online 2013 Oct 18. doi: 10.1073/pnas.1316981110

2. Chen, G., Swem, L.R., Swem, D.L., Stauff, D.L., O’Loughlin, C.T., Jeffrey, P.D., Bassler, B.L., Hughson, F.M., A strategy for antagonizing quorum sensing. Mol Cell. 2011 Apr 22; 42(2): 199–209. doi: 10.1016/j.molcel.2011.04.003

1. Swem, L.R., Swem, D.L., O’Loughlin, C.T., Gatmaitan, R., Zhao, B., Ulrich, S.M., Bassler, B.L., A Quorum-Sensing Antagonist Targets Both Membrane-Bound and Cytoplasmic Receptors And Controls Bacterial Pathogenicity. Mol Cell. 2009 Jul 31; 35(2): 143–153. doi: 10.1016/j.molcel.2009.05.029