{"id":5500,"date":"2023-08-01T08:17:26","date_gmt":"2023-08-01T12:17:26","guid":{"rendered":"https:\/\/www.bates.edu\/faculty\/profile\/daniel-slane\/"},"modified":"2026-02-04T16:08:44","modified_gmt":"2026-02-04T21:08:44","slug":"daniel-slane","status":"publish","type":"faculty-profile","link":"https:\/\/www.bates.edu\/faculty\/profile\/daniel-slane\/","title":{"rendered":"Daniel Slane"},"content":{"rendered":"

Ph.D. in Biology, <\/strong>MPI for Biology \/ University of T\u00fcbingen (Germany)<\/strong><\/p>\n

Office hours: Tuesday, 10 – 11 a.m.<\/p>\n

I am currently not accepting new requests for letters of recommendation.<\/p>\n

Research Interests<\/h6>\n

I am broadly interested in how plants on a cell-to-cell level process external information that affects their development. More specifically, I want to understand how this information in the form of abiotic stresses such as heat or drought is interpreted on a genomic level at various stages of development. My research primarily focuses on understanding how this information is stored and encoded in the context of chromatin and 3-dimensional contacts of DNA and its associated proteins inside the nucleus so that plants “learn” how to adapt to recurring stress situations. In my lab, we use next-generation sequencing, plant physiological, genetic, molecular and cell biology approaches to tackle these questions. Especially in light of climate change, it will be important to understand how plants react to changing environmental conditions.<\/p>\n

Publications<\/h4>\n

Chen H, Xiong F, Wangler AM, Bischoff T, Wang K, Miao Y, Slane D, Schwab R, Laux T, and Bayer M.<\/strong> Phosphorylation-Dependent Activation of the bHLH Transcription Factor ICE1\/SCRM Promotes Polarization of the Arabidopsis Zygote. New Phytologist<\/em>. 2025<\/strong> Feb;245(3):1029-1039. doi: 10.1111\/nph.20265. Epub 2024 Nov 14.<\/span> PMID: 39543803.
\nhttps:\/\/doi.org\/10.1111\/nph.20265<\/a><\/p>\n

Berendzen KW, Grefen Christopher, Sakamoto Takuya, and Slane D.<\/strong> Analysis of Chromatin Accessibility, Histone Modifications, and Transcriptional States in Specific Cell Types Using Flow Cytometry. Methods in Molecular Biology<\/em>. 2023<\/strong>, 2698:57-73.
\nhttps:\/\/doi.org\/10.1007\/978-1-0716-3354-0_5<\/a><\/p>\n

Sakamoto T, Sakamoto Y, Grob S, Slane D, Yamashita T, Ito N, Oko Y, Sugiyama T, Higaki T, Hasezawa S, Tanaka M, Matsui A, Seki M, Suzuki T, Grossniklaus U, and Matsunaga S<\/span>.<\/strong> Two-step regulation of centromere distribution by condensin II and the nuclear envelope proteins. Nature Plants<\/em>. 2022<\/strong> Aug;8(8):940-953.
\nhttps:\/\/doi.org\/10.1038\/s41477-022-01200-3<\/a><\/p>\n

Slane D, Lee CH, Kolb M, Dent C, Miao Y, Franz-Wachtel M, Lau S, Ma\u010dek B, Balasubramanian S, Bayer M, and J\u00fcrgens G<\/span>.<\/strong> The integral spliceosomal component CWC15 is required for development in Arabidopsis. Scientific Reports<\/em>. 2020<\/strong> Aug 7;10(1):13336.
\nhttps:\/\/doi.org\/10.1038\/s41598-020-70324-3<\/a><\/p>\n

Smit ME, Llavata-Peris CI, Roosjen M, van Beijnum H, Novikova D, Levitsky V, Sevilem I, Roszak P, Slane D, J\u00fcrgens G, Mironova V, Brady SM, and Weijers D.<\/span><\/strong> Specification and regulation of vascular tissue identity in the Arabidopsis<\/em> embryo. Development<\/em>. 2020<\/strong> Apr 20;147(8):dev186130.
\nhttps:\/\/doi.org\/10.1242\/dev.186130<\/a><\/p>\n

Slane D<\/strong>, Berendzen KW, Witth\u00f6ft J, and J\u00fcrgens G<\/strong>.<\/span> Transcriptomic Profiling of the Arabidopsis Embryonic Epidermis Using FANS in Combination with RNAseq. Methods in Molecular Biology<\/em>. 2020<\/strong>;2122:151-164.
\nhttps:\/\/doi.org\/10.1007\/978-1-0716-0342-0_12<\/a><\/p>\n

Neu A, Eilbert E, Asseck LY, Slane D, Henschen A, Wang K, B\u00fcrgel P, Hildebrandt M, Musielak TJ, Kolb M, Lukowitz W, Grefen C, and Bayer M.<\/span><\/strong> Constitutive signaling activity of a receptor-associated protein links fertilization with embryonic patterning in Arabidopsis thaliana<\/em>. Proceedings of the National Academy of Sciences of the United States of America<\/em>. 2019<\/strong> Mar 19;116(12):5795-5804.
\nhttps:\/\/doi.org\/10.1073\/pnas.1815866116<\/a><\/p>\n

Wallmeroth N, Jeschke D, Slane D, N\u00e4gele J, Veerabagu M, Mira-Rodado V, and Berendzen KW.<\/span><\/strong> ARR22 overexpression can suppress plant Two-Component Regulatory Systems. PLoS ONE<\/em>. 2019<\/strong> Feb 11;14(2):e0212056.
\nhttps:\/\/doi.org\/10.1371\/journal.pone.0212056<\/a><\/p>\n

Slane D, Reichardt I, El Kasmi F, Bayer M, and J\u00fcrgens G<\/span>.<\/strong> Evolutionarily diverse SYP1 Qa-SNAREs jointly sustain pollen tube growth in Arabidopsis. The Plant Journal<\/em>. 2017<\/strong> Nov;92(3):375-385.
\nhttps:\/\/doi.org\/10.1111\/tpj.13659<\/a><\/p>\n

Slane D, B\u00fcrgel P, and Bayer M.<\/strong> Staining and Clearing of Arabidopsis Reproductive Tissue for Imaging of Fluorescent Proteins. Methods in Molecular Biology<\/em>. 2017<\/strong>;1669:87-94.
\nhttps:\/\/doi.org\/10.1007\/978-1-4939-7286-9_8<\/a><\/p>\n

Slane D and Bayer M.<\/strong> Cell Type-Specific Gene Expression Profiling Using Fluorescence-Activated Nuclear Sorting. Methods in Molecular Biology<\/em>. 2017<\/strong>;1629:27-35.
\nhttps:\/\/doi.org\/10.1007\/978-1-4939-7125-1_3<\/a><\/p>\n

Bayer M, Slane D, and J\u00fcrgens G.<\/strong> Early plant embryogenesis-dark ages or dark matter? Current Opinion in Plant Biology<\/em>. 2017<\/strong> Feb;35:30-36.
\nhttps:\/\/doi.org\/10.1016\/j.pbi.2016.10.004<\/a><\/p>\n

Musielak TJ, Slane D, Liebig C, and Bayer M.<\/span><\/strong> A Versatile Optical Clearing Protocol for Deep Tissue Imaging of Fluorescent Proteins in Arabidopsis thaliana<\/em>. PLoS ONE<\/em>. 2016<\/strong> Aug 12;11(8):e0161107.
\nhttps:\/\/doi.org\/10.1371\/journal.pone.0161107<\/a><\/p>\n

Murphy E, Vu LD, Van den Broeck L, Lin Z, Ramakrishna P, van de Cotte B, Gaudinier A, Goh T,<\/strong> Slane D<\/b>, Beeckman T, Inz\u00e9 D, Brady SM, Fukaki H, De Smet I.<\/strong><\/span> RALFL34 regulates formative cell divisions in Arabidopsis pericycle during lateral root initiation. Journal of Experimental Botany<\/em>. 2016<\/strong> Aug;67(16):4863-4875.
\nhttps:\/\/doi.org\/10.1093\/jxb\/erw281<\/a><\/p>\n

Slane D, Kong J, Schmid M, J\u00fcrgens G, Bayer M<\/span>.<\/strong> Profiling of embryonic nuclear vs. cellular RNA in Arabidopsis thaliana<\/em>. Genomics Data<\/em>. 2015<\/strong> Apr 8;4:96-98.
\nhttps:\/\/doi.org\/10.1016\/j.gdata.2015.03.015<\/a><\/p>\n

Slane D, Kong J, Berendzen KW, Kilian J, Henschen A, Kolb M, Schmid M, Harter K, Mayer U, De Smet I, Bayer M, J\u00fcrgens G.<\/span><\/strong> Cell type-specific transcriptome analysis in the early Arabidopsis thaliana<\/em> embryo. Development<\/em>. 2014<\/strong> Dec;141(24):4831-4840.
\nhttps:\/\/doi.org\/10.1242\/dev.116459<\/a><\/p>\n

Lau S, Slane D, Herud O, Kong J, J\u00fcrgens G.<\/span><\/strong> Early Embryogenesis in Flowering Plants: Setting Up the Basic Body Pattern. Annual Review of Plant Biology<\/em>. 2012<\/strong>;63:483-506.
\nhttps:\/\/doi.org\/10.1146\/annurev-arplant-042811-105507<\/a><\/p>\n

Reichardt I,<\/strong> Slane D<\/b>, El Kasmi F, Kn\u00f6ll C, Fuchs R, Mayer U, Lipka V, J\u00fcrgens G.<\/strong><\/span> Mechanisms of Functional Specificity Among Plasma-Membrane Syntaxins in Arabidopsis<\/em>.\u00a0Traffic<\/em>.\u00a02011<\/strong> Sep;12(9):1269-1280.
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