{"id":636,"date":"2017-10-15T23:00:11","date_gmt":"2017-10-15T23:00:11","guid":{"rendered":"https:\/\/www.bates.edu\/williams-lab\/?p=636"},"modified":"2024-01-04T16:49:22","modified_gmt":"2024-01-04T21:49:22","slug":"suddenly-its-mid-october","status":"publish","type":"post","link":"https:\/\/www.bates.edu\/williams-lab\/2017\/10\/15\/suddenly-its-mid-october\/","title":{"rendered":"Suddenly it\u2019s mid-October??"},"content":{"rendered":"

It\u2019s so crazy that we are almost halfway through fall semester. <\/p>\n

A full year thesis used to seem so long to me\u2014now I\u2019m thinking it\u2019s not nearly enough time to get what I want to get done, done. I\u2019ve been trying to amplify alas2<\/em> so that I can get it into a plasmid and move forward with the gain of function assay. My issue right now is that my primers are amplifying all the wrong stuff!! We\u2019re getting lots of short strands of amplified material, and are not seeing anything large enough to be the alas2 gene. We tried PCR with both cDNA and gDNA, with similar results (gDNA gel below\u2014we wanted to see a band significantly larger than the largest ladder marker, but haven\u2019t yet). I am still loving working independently in the lab, and problem-solving the issues I have faced thus far (we ran out of gDNA and I isolated it all on my own last week!), but am realizing that science takes a lot longer than I thought. But it\u2019s worth it. Because when things do start to go right it\u2019s going to be so cool! And advance scientific though in this field!!
\n\"gel\"<\/p>\n

Anyways, in other news, we had a wonderful presentation by Gwen on Wednesday on transcription factors NF-E2, FLI1, and RUNX1 and their roles in mature mouse megakaryocytes. The figures in the paper were very detailed and I learned more on some unfamiliar\/rusty lab techniques including gene ontology analysis, ChIP, and flow cytometry! <\/p>\n

Gwen\u2019s Western blots are going better now that she figured out that there was an issue with the SDS she was using (thankfully it wasn\u2019t the gel–those things are probably expensive). Mel and Jason are killing it with the microinjections and are refining their techniques. Honestly microinjecting seems so hard they are seriously #true #scientists. Mel also is working on the confocal and working out the kinks-woohoo! We also got videotaped for a video essay Larissa needs to submit for a Women in Science grant!! We are #movie #stars. That\u2019s all for now, happy October break!<\/p>\n","protected":false},"excerpt":{"rendered":"

It\u2019s so crazy that we are almost halfway through fall semester. A…<\/p>\n","protected":false},"author":381,"featured_media":637,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_hide_ai_chatbot":false,"_ai_chatbot_style":"","associated_faculty":[],"_Page_Specific_Css":"","_bates_restrict_mod":false,"_batesModPostContentOverride_prepend":false,"_batesModPostContentOverride_append":false,"_batesModPostContentOverride_append_before_footer":false,"_table_of_contents_display":false,"_table_of_contents_location":"","_table_of_contents_disableSticky":false,"_is_featured":false,"footnotes":"","_bates_seo_meta_description":"","_bates_seo_block_robots":false,"_bates_seo_sharing_image_id":0,"_bates_seo_sharing_image_twitter_id":0,"_bates_seo_share_title":"","_bates_seo_canonical_overwrite":"","_bates_seo_twitter_template":""},"categories":[1],"tags":[],"class_list":["post-636","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog"],"_links":{"self":[{"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/posts\/636","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/users\/381"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/comments?post=636"}],"version-history":[{"count":1,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/posts\/636\/revisions"}],"predecessor-version":[{"id":638,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/posts\/636\/revisions\/638"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/media\/637"}],"wp:attachment":[{"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/media?parent=636"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/categories?post=636"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/tags?post=636"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}