{"id":639,"date":"2017-10-27T15:25:32","date_gmt":"2017-10-27T15:25:32","guid":{"rendered":"https:\/\/www.bates.edu\/williams-lab\/?p=639"},"modified":"2024-01-04T16:42:42","modified_gmt":"2024-01-04T21:42:42","slug":"thesis-is-getting-real","status":"publish","type":"post","link":"https:\/\/www.bates.edu\/williams-lab\/2017\/10\/27\/thesis-is-getting-real\/","title":{"rendered":"Thesis is Getting Real."},"content":{"rendered":"<p>Wow! It\u2019s hard to believe we are already a quarter of the way through our senior year (and thesis!). <\/p>\n<p>We got back from October break this week, and it seems like a lot of seniors are starting to feel that thesis stress. It\u2019s crazy that some of my friends who are doing half a year thesis have theirs due in about a month! <\/p>\n<p>It\u2019s been another busy week in the Williams lab. Mel and Jason have been working hard at microinjecting and have both had some success injecting morpholinos! Rachel is hard at work trying to figure out her PCR conditions, but is making progress. <\/p>\n<p>I have been busy running western blots. Before break and after break, I found Nfe2 in zebrafish embryos! This is really exciting, as nobody has done it before. Now, I am running a western on a time series of embryos that a past thesis student collected and prepped. We saw Nfe2 now we are able to get a better idea of when it is expressed during development.<br \/>\n<div id=\"attachment_640\" style=\"width: 410px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-640\" src=\"https:\/\/www.bates.edu\/williams-lab\/files\/2017\/10\/Nfe2-expression-400x77.png\" alt=\"\" width=\"400\" height=\"77\" class=\"size-medium wp-image-640\" srcset=\"https:\/\/www.bates.edu\/williams-lab\/files\/2017\/10\/Nfe2-expression-400x77.png 400w, https:\/\/www.bates.edu\/williams-lab\/files\/2017\/10\/Nfe2-expression-768x148.png 768w, https:\/\/www.bates.edu\/williams-lab\/files\/2017\/10\/Nfe2-expression-900x173.png 900w, https:\/\/www.bates.edu\/williams-lab\/files\/2017\/10\/Nfe2-expression-200x38.png 200w, https:\/\/www.bates.edu\/williams-lab\/files\/2017\/10\/Nfe2-expression.png 1919w\" sizes=\"(max-width: 400px) 100vw, 400px\" \/><p id=\"caption-attachment-640\" class=\"wp-caption-text\">Nfe2 expression in zebrafish&#8211;each well is a different developmental time point (moving from youngest to oldest)<\/p><\/div><br \/>\nIt\u2019s been slow going, but I feel like I\u2019m finally making some progress and I\u2019m excited to get going on ChIP soon!<\/p>\n<p>At this week\u2019s lab meeting, Jason gave a great presentation on his project, covering background and methods. I really enjoyed hearing about what he\u2019s been up to in lab, and some of the background is useful for helping to put my project into context and to get a better perspective on the larger goals of our lab. I\u2019m looking forward to Rachel\u2019s and Mel\u2019s presentations in the coming weeks!<\/p>\n<p>Lastly, we\u2019ve all been reading and prepping for our intros, which are due in three weeks! It\u2019s kind of scary and stressful, but I\u2019m trying to break it into smaller sections and work on it for a little each day to make it more manageable. I know all the hard work will pay off in the long run, and it will feel great to have a draft written before Thanksgiving. <\/p>\n<p>We\u2019re back from break and thesis is ramping up, but we\u2019re ready for it!<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Wow! 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