Things have been picking up for all of us as the semester seems to accelerate towards Thanksgiving break. <\/p>\n
We have all been working hard both on our lab projects themselves, and on our Introductions, which are due right before the break. So much to do, and so little time!<\/p>\n
I have been injecting embryos with a control morpholino, dosing them with either MEHP (a phthalate) or DMSO (the solvent, and thus a control), and then scoring various aspects of their development. I feel increasingly comfortable scoring things such as heart and jaw development, and it has been great to see noticeable progress in my ability to spot abnormal phenotypes. I have also finally mastered viewing fast-moving fish at the 72 and 96 hour time points, the source of some previous frustration! I have also become increasingly comfortable trusting my observations, especially as I have begun blinding myself to which treatment group is which. Interestingly, I have seen several instances of pericardial edema and enlarged hearts in both the treatment and control group thus far! Next week I will begin collecting a full set of data with 15 fish in each treatment group \u2013 I can\u2019t wait to get a full set of data!<\/p>\n
The things we do for science<\/p>\n
Gwen had a particularly late night\/early morning this week, venturing into the maze of Carnegie at 2 am in order to freeze embryos at 18 hours post fertilization. She has been working hard all week collecting embryos for a \u201ctime series\u201d, in which she will use Western Blotting to examine Nfe2 levels at different time points during development. Rachel has been working hard on designing a new set of primers for alas2<\/em>, while Mel has been practicing microinjecting (with great success!) and taking images of cilia in zebrafish otic vesicles on the confocal microscope.<\/p>\n We all seem to be hitting our strides, with a clear idea of what our lab projects will entail, and a plan to get there!<\/p>\n","protected":false},"excerpt":{"rendered":" Things have been picking up for all of us as the semester…<\/p>\n","protected":false},"author":381,"featured_media":559,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_hide_ai_chatbot":false,"_ai_chatbot_style":"","associated_faculty":[],"_Page_Specific_Css":"","_bates_restrict_mod":false,"_batesModPostContentOverride_prepend":false,"_batesModPostContentOverride_append":false,"_batesModPostContentOverride_append_before_footer":false,"_table_of_contents_display":false,"_table_of_contents_location":"","_table_of_contents_disableSticky":false,"_is_featured":false,"footnotes":"","_bates_seo_meta_description":"","_bates_seo_block_robots":false,"_bates_seo_sharing_image_id":0,"_bates_seo_sharing_image_twitter_id":0,"_bates_seo_share_title":"","_bates_seo_canonical_overwrite":"","_bates_seo_twitter_template":""},"categories":[1],"tags":[],"class_list":["post-642","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog"],"_links":{"self":[{"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/posts\/642","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/users\/381"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/comments?post=642"}],"version-history":[{"count":1,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/posts\/642\/revisions"}],"predecessor-version":[{"id":643,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/posts\/642\/revisions\/643"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/media\/559"}],"wp:attachment":[{"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/media?parent=642"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/categories?post=642"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/tags?post=642"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}