{"id":645,"date":"2017-11-09T23:18:43","date_gmt":"2017-11-09T23:18:43","guid":{"rendered":"https:\/\/www.bates.edu\/williams-lab\/?p=645"},"modified":"2024-01-04T16:47:07","modified_gmt":"2024-01-04T21:47:07","slug":"get-ready-to-write","status":"publish","type":"post","link":"https:\/\/www.bates.edu\/williams-lab\/2017\/11\/09\/get-ready-to-write\/","title":{"rendered":"Get Ready to Write!"},"content":{"rendered":"<p>With just one week before Thanksgiving, all of us in lab have been kicking it into high gear and churning out our first drafts of our introductions! It has been a flurry of reading, writing, and citing, but we have all been doing a great job of keeping it up!<\/p>\n<p>In addition to our introductions, all of us have been hard at work getting into the lab before we leave. I have been continuing to work on my microinjecting skills, and I have gotten much better! I have been using the control morpholino to practice in an attempt to get my success rate up to 80%, so hopefully I will soon be able to move onto the nfe2-MO! Then I can move onto the task of creating scoring criteria for assessing the zebrafish cilia and neuromasts when nfe2 is knocked down. I have also made a lot of progress on imaging with the confocal, which is how I will be collecting data on the morphant fish.<\/p>\n<p>In other news, Rachel received her primers this week! She eluted them and ran a PCR on genomic DNA in order to amplify <em>alas2<\/em>. Gwen has been a champion of the lab, going in at all hours of the day and night to collect embryos for her time series, which she is now using to run a Western blot. Jason has continued to dose his embryos with MEHP, and he has been scoring the resultant phenotypes. <\/p>\n<p>All in all, it&#8217;s been a crazy busy week, and I think we will all be grateful to be eating some turkey really soon. <\/p>\n<p>Here is an exciting confocal picture of a cluster of hair cells:<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.bates.edu\/williams-lab\/files\/2017\/11\/hair-300x300.jpg\" alt=\"\" width=\"300\" height=\"300\" class=\"alignnone size-medium wp-image-646\" srcset=\"https:\/\/www.bates.edu\/williams-lab\/files\/2017\/11\/hair-300x300.jpg 300w, https:\/\/www.bates.edu\/williams-lab\/files\/2017\/11\/hair-150x150.jpg 150w, https:\/\/www.bates.edu\/williams-lab\/files\/2017\/11\/hair-200x200.jpg 200w, https:\/\/www.bates.edu\/williams-lab\/files\/2017\/11\/hair.jpg 640w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>With just one week before Thanksgiving, all of us in lab have&hellip;<\/p>\n","protected":false},"author":381,"featured_media":646,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_hide_ai_chatbot":false,"_ai_chatbot_style":"","associated_faculty":[],"_Page_Specific_Css":"","_bates_restrict_mod":false,"_batesModPostContentOverride_prepend":false,"_batesModPostContentOverride_append":false,"_batesModPostContentOverride_append_before_footer":false,"_table_of_contents_display":false,"_table_of_contents_location":"","_table_of_contents_disableSticky":false,"_is_featured":false,"footnotes":"","_bates_seo_meta_description":"","_bates_seo_block_robots":false,"_bates_seo_sharing_image_id":0,"_bates_seo_sharing_image_twitter_id":0,"_bates_seo_share_title":"","_bates_seo_canonical_overwrite":"","_bates_seo_twitter_template":""},"categories":[1],"tags":[],"class_list":["post-645","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog"],"_links":{"self":[{"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/posts\/645","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/users\/381"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/comments?post=645"}],"version-history":[{"count":1,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/posts\/645\/revisions"}],"predecessor-version":[{"id":647,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/posts\/645\/revisions\/647"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/media\/646"}],"wp:attachment":[{"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/media?parent=645"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/categories?post=645"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bates.edu\/williams-lab\/wp-json\/wp\/v2\/tags?post=645"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}