
Daniel Slane
Visiting Assistant Professor of Biology
Associations
Biology
Carnegie Science Hall, Room 414
About
Ph.D. in Biology, MPI for Biology / University of Tübingen (Germany)
Research Interests
I am broadly interested in how plants on a cell-to-cell level process external information that affects their development. More specifically, I want to understand how this information in the form of abiotic stresses such as heat or drought is interpreted on a genomic level at various stages of development. My research primarily focuses on understanding how this information is stored and encoded in the context of chromatin and 3-dimensional contacts of DNA and its associated proteins inside the nucleus so that plants “learn” how to adapt to recurring stress situations. In my lab, we use next-generation sequencing, plant physiological, genetic, molecular and cell biology approaches to tackle these questions. Especially in light of climate change, it will be important to understand how plants react to changing environmental conditions.
Publications
Chen H, Xiong F, Wangler AM, Bischoff T, Wang K, Miao Y, Slane D, Schwab R, Laux T, and Bayer M. Phosphorylation-Dependent Activation of the bHLH Transcription Factor ICE1/SCRM Promotes Polarization of the Arabidopsis Zygote. New Phytologist. 2024 Nov 14; Epub ahead of print. PMID: 39543803.
https://doi.org/10.1111/nph.20265
Berendzen KW, Grefen Christopher, Sakamoto Takuya, and Slane D. Analysis of Chromatin Accessibility, Histone Modifications, and Transcriptional States in Specific Cell Types Using Flow Cytometry. Methods in Molecular Biology. 2023, 2698:57-73.
https://doi.org/10.1007/978-1-0716-3354-0_5
Sakamoto T, Sakamoto Y, Grob S, Slane D,
https://doi.org/10.1038/s41477-022-01200-3
Slane D,
https://doi.org/10.1038/s41598-020-70324-3
Smit ME, Llavata-Peris CI, Roosjen M, van Beijnum H, Novikova D, Levitsky V, Sevilem I, Roszak P, Slane D,
https://doi.org/10.1242/dev.186130
Slane D
https://doi.org/10.1007/978-1-0716-0342-0_12
https://doi.org/10.1073/pnas.1815866116
https://doi.org/10.1371/journal.pone.0212056
https://doi.org/10.1111/tpj.13659
Slane D, Bürgel P, and Bayer M. Staining and Clearing of Arabidopsis Reproductive Tissue for Imaging of Fluorescent Proteins. Methods in Molecular Biology. 2017;1669:87-94.
https://doi.org/10.1007/978-1-4939-7286-9_8
Slane D and Bayer M. Cell Type-Specific Gene Expression Profiling Using Fluorescence-Activated Nuclear Sorting. Methods in Molecular Biology. 2017;1629:27-35.
https://doi.org/10.1007/978-1-4939-7125-1_3
Bayer M, Slane D, and Jürgens G. Early plant embryogenesis-dark ages or dark matter? Current Opinion in Plant Biology. 2017 Feb;35:30-36.
https://doi.org/10.1016/j.pbi.2016.10.004
https://doi.org/10.1371/journal.pone.0161107
https://doi.org/10.1093/jxb/erw281
https://doi.org/10.1016/j.gdata.2015.03.015
https://doi.org/10.1242/dev.116459
Expertise
Current Courses
Fall Semester 2025
Introduction to Biotechnology – Concepts, Advantages, and Risks
At the heart of Biotechnology lies the manipulation of living cells or organisms in order to perform a specific, predictable task used to solve problems and produce commercial products. It has therefore a wide range of applications in the medical and pharmaceutical, agricultural, environmental, or c…
Genetics
Genetics is the study of information transfer across generations. In this course, students examine the molecular basis of genetic information, consider the consequences of mutations, identify common patterns of inheritance, apply probability and statistics to understand genetic problems, and learn a…
Senior Thesis
Permission of the department and the thesis advisor are required. Students register for BIO 457 in the fall semester. Majors writing an honors thesis register for both BIO 457 and 458.