Microscopy

Bates supports a wide variety of microscopy techniques (see below). These instruments are available to all Bates faculty, staff, and students. For training or general consultation, please contact Mark Lessard.

+JEOL JSM-7100FLV

Field Emission Variable Vacuum Scanning Electron Microscope with EDS

This instrument is equipped with a sample chamber-level secondary electron detector and both HV and LV backscatter detectors. It is equipped with a Schottkey thermal field emission gun and offers resolution of approximately 2-3 nm at 30 kV.

The SEM is equipped with a Thermo/ Noran System 7 Energy Dispersive Spectrometer with a LN-free digital detector. This system enables quantitative spectrum analysis with and without standards, spectral imaging, x-ray mapping, x-ray line scans, point and shoot sampling, and digital image acquisition.

Sample preparation equipment for the SEM:

Denton Vacuum DV1 Carbon Evaporation unit; attachments for rods or yarn 

Anatech Hummer 6.6 Sputtering System with rotating, tilted stage and Au/Pd target 

Ladd Research CO2 Critical Point Dryer 

Scandium Image analysis software

Adobe Photoshop software

Fume hood

+Leica SP8 Laser Scanning Confocal Microscope

The Leica SP8 confocal microscope is equipped with the following:

  • 405nm laser, Argon laser (458, 476, 488, 496, 514nm lines), White Light Laser (470nm -670nm), excitation sources
  • 5 fluorescence detectors (2 PMT’s, 3 HyD’s), and a transmitted light detector
  • Objectives:
    • 10x 0.35NA
    • 20x 0.75NA multi-immersion
    • 25x 0.95NA water immersion 
    • 40x 1.1NA water immersion with motorized correction collar
    • 40x 1.3NA oil immersion
    • 63x 1.4NA oil immersion
  • Full environmental chamber for live imaging
+Other light microscopes available:

Nikon Eclipse TE2000-U Widefield Fluorescence Scope (Inverted)

Configuration:

  • Motorized stage, focus, and shutters
  • Excite 120 fluorescence light source
  • 5 filter cube positions
  • Nikon Digital Sight DS-Qi1Mc Camera (monochrome, 1.5 megapixel, cooled)
  • Objectives:
    • 4x 0.13 NA Plan Fluor
    • 10x 0.3 NA ELWD Plan Fluor
    • 20x 0.45 NA ELWD Plan Fluor
    • 40x 0.6 NA ELWD Plan Fluor

Nikon Eclipse 80i Fluorescence Scope (Upright)

Configuration:

  • Automated stage and focus
  • 6 filter cube positions
  • XCite 120 Fluorescent Source
  • QImaging Retiga EXi Camera (mono and RGB capable)
  • Objectives:
    • 4x 0.2 NA Plan APO
    • 10x 0.45 NA Plan APO
    • 20x 0.7 NA Plan APO
    • 40x 0.95 NA Plan APO

Nikon Modular Focus Scope (Upright)

  • Configuration:
    • Automated stage and focus
    • Nikon Digital Sight DS-Ri1 Camera
    • 100w Halogen BF source
    • XCite 120 Fluorescence Source
    • FiberLite Transillumination source
    • FiberLite Gooseneck source
    • Objectives:
      • 2x 0.1 NA Plan APO
      • 5x 0.15 NA LU Plan Fluor
      • 10x 0.3 NA LU Plan Fluor
      • 20x 0.4 NA ELWD LU Plan
      • 50x 0.55 NA ELWD LU Plan
  • Notes: 
    • This is a unique system that crosses a stand from a dissection scope with the imaging head of a standard upright scope.
    • It is capable of fluorescence, brightfield, darkfield, DIC, Phase, and Polarization